May depend on the instrument and/or the user's preferences.
May still require lessons and practice.
The Devil (Tenacious D and the Pick of Destiny)
Todd Ingram (Scott Pilgrim vs. The World)
Kyle & Ken Katayanagi (Scott Pilgrim vs. The World)
Sasha La Fleur (All Dogs Go To Heaven)
The Singin Strays (The Fox and The Hound II)
Duchess (The Aristocats)
Marie (The Aristocats)
Toulouse (The Aristocats)
Scat Cat and his gang (The Aristocats)
The Chipmunks (Alvin and the Chipmunks)
The Chipettes (Alvin and the Chipmunks)
Rainbooms (My Little Pony Equestria Girls)
Sirens/The Dazzlings (My Little Pony Equestria Girls)
Songbird Serenade (My Little Pony: The Movie)
Various Musicians (Rock Dog)
Robot Devil (Futurama)
DJ Pon-3 (My Little Pony: Friendship Is Magic)
Jagged Stone/Guitar Villain (Miraculous Ladybug)
Greg Universe (Steven Universe)
Steven Universe (Steven Universe)
Sumalee & Yo Yo (Pound Puppies)
Ember McLain (Danny Phantom)
Luna Loud (The Loud House)
Zoe Trent (Littlest Pet Shop)
Todd Ingram (Scott Pilgrim)
Music Meister (DC Comic)
Live Action TV
The Minstrel (Batman 60s series)
Pumpkin Rapper (Mighty Morphin Power Rangers)
Hate Master (Mighty Morphin Power Rangers)
Musician (Valkyrie Crusade)
Johnny (Devil Went Down to Georgia)
The Devil (Devil Went Down to Georgia)
Ninja Brian (Ninja Sex Party)
Miku Izayoi (Date A Live)
Brook (One Piece)
Pick of Destiny (Tenacious D and the Pick of Destiny)
The Devil (Tenacious D and the Pick of Destiny) is skilled with a number of rocking tools, including singing, drums and electric guitar.
Combining his bass playing with his vegan powers, Todd Ingram (Scott Pilgrim vs. the World) can send out powerful shockwaves.
The Katayanagi Twins (Scott Pilgrim) uses their magical keyboard to summon twin dragons to attack the opposing band.
The Minstrel (Batman 60s series) was an electronic genius which was in fact a logical pursuit for someone who was also highly talented in music since both music and electronic energy are transmitted in waves.
The Robot Devil (Futurama) is able to masterfully play three bows on a solid-gold fiddle at once.
As a world-famous rockstar, Jagged Stone (Miraculous Ladybug) has a natural talent for music...
...a skill carried over in his akumatized persona Guitar Villain.
Having once been a rock and roll one-man band, Greg Universe (Steven Universe) is adept in the use of multiple instruments...
...a skill carried on in his son Steven Universe.
The Devil (Devil Went Down to Georgia) makes bets with fiddlers on their skill, winning their souls should he win.
Johnny (Devil Went Down to Georgia) is so skilled on the violin that he beat the Devil in a fiddling contest, winning a fiddle of gold in the process.
Ninja Brian (Ninja Sex Party) is proficient in a number of instruments, and able to play the piano, pan-flute, maracas and a gong all at the same time.
Combining his bass playing with his vegan powers, Todd Ingram (Scott Pilgrim) can send out powerful shockwaves.
Musician (Valkyrie Crusade) is a so skilled musician that she is loved even by the god of music himself.
The Chipmunks & The Chipettes (Alvin and the Chipmunks)
Gazelle and the Tiger Dancers (Zootopia)
Bodi (Rock Dog) is a talented guitar play, even at a beginner's level, after being taught by Angus, he was able to save Snow Mountain and become co-guitarist of his band.
Angus Scattergood (Rock Dog) is a famous rock legend that specializes in rock music.
Darma (Rock Dog)
Germur (Rock Dog)
Fleetwood Yak (Rock Dog) was once a drummer in the city, however, when playing in Bodi's band, he retains his specialty.
Trey (Rock Dog)
Miku Izayoi (Date A Live) is a extremely talented singer.
Pumpkin Rapper (Mighty Morphin Power Rangers) would use his clever raps and rhymes to distract his opponents.
Sasha La Fleur (All Dogs Go To Heaven Series)
Brook (One Piece) is a virtuoso musician highly skilled in playing all kinds of instruments such as the violin...