Genryūsai Shigekuni Yamamoto (Bleach) sacrificed his arm to activate "Hadō 96: Ittō Kasō".
The DigiDestined (Digimon series) utilized their crests together to create the ultimate protection over the Digital World from foreign evils outside their planetary systems, but because of that their Digimon will not be able to transform into their ultimate forms ever again.
Aqua (Kingdom Hearts) sacrificed her Keyblade and armor to save Terra-Xehanort and get him back to the Realm of Light, falling into darkness as a result.
Calamity Ganon (The Legend of Zelda: Breath of the Wild) completely shed his mortal form and abandoned his attempt to reincarnate in exchange for power, becoming Dark Beast Ganon, a creature of pure hatred and malice.
Chiyo (Naruto) sacrificing her own life to revive Gaara.
Orochimaru (Naruto) sacrificing the four White Zetsu victims to resurrect the first four Hokage.
After gaining 99 extra lives, Mario (Super Mario-Kun) sacrificed all of those extra lives to create clones of himself.
Midora (Toriko) sacrificed himself to heal the entire planet.
"Ectoplasmer" (Yu-Gi-Oh!) is a Magic Card that allows the user to sacrifice their own monsters to attack the opponent directly.
Konosuke Ōshuta (Yu-Gi-Oh!) using his Deck Master Deepsea Warrior's ability, "Reflector Hole", sacrificing two of his own monsters to deflect his opponent's attack back at them.