Tenjian's Ultimate Skill (Azure Striker Gunvolt 2), Seven Slashes bypasses the Conditional Invulnerability and will kill him once finished
Chaozu (Dragon Ball Z) self-destructs in a failed attempt to take Nappa with him.
Tenshinhan (Dragon Ball Z) using the Shin Kikōhō/Neo Tri-Beam, a very draining attack that can eventually kill him if used at a low reserve, as he once died doing so.
Semi-Perfect Cell (Dragon Ball Z) activating his planet-destroying self-destruction that even he cannot stop.
Vegeta (Dragon Ball Z) releasing all the energy within his body for a gigantic explosion at the cost of his own life.
Tempesta (Fairy Tail) can "die once" to create a anti-magic mist that kills Mages and non-magic beings.
Once defeated, a Voltigore’s (Hλlf-Life: Opposing Force) carapace that stores all the biological electricity will deteriorate and explodes along with the corpse, causing bio-electricity and poisonous-acidic damage.
Axel (Kingdom Hearts series) ends up destroying himself by putting all of his power into a single attack that destroys every other Nobody in the general vicinity.
Deidara (Naruto) using C0 the highest form of his explosive clay attacks which he becomes a suicide-bomb as a last resort that covers ten kilometer blast radius.
The destructive power of a Reject Dial (One Piece) is equally inflicted on both the target and the user. Just one use of the dial is enough to kill a normal person.
Black Fog (Pokémon) using Self-Destruct as its last resort, exploding and killing itself rather than being captured by a human.
The Soldier (Team Fortress 2), when equipped with the Equalizer, can perform the kamikaze taunt which allows him to kill himself and any enemies nearby.